1. Introduction to the Quantitative Biochemistry Laboratory
Laboratory safety, error analysis and pipetting
techniques
2. Colorimetry - A Simple Spectrophotometric Approach to Quantitative
Analysis
Defining relationships between color and amounts
by analyzing p-nitrophenol which serves as the chromophore in activity
assays of alkaline phosphatase and proteases; Application of the
Beer-Lambert Law A = e x
l
x C; To examine the relationship between absorbance and concentration
for dilute solutions, and to determine the molar absorptivity e
(or extinction coefficient, cm–1 M–1)
of the compound at certain wavelength.
3. Quantifying Biological Macromolecules using Spectroscopy with
Focus on Proteins
Determination of unknown protein concentration by
means of Bradford Protein Assay and Bicinchoninic Acid Assay
4. Enzymology - Biological and Technological Significance
Studying enzyme kinetics by using the enzymes alkaline phosphatase
and proteases as examples; to explore the effect of substrate and inhibitor
concentration on enzyme function
5. Principles of Chromatography: Separation of Biological molecules
Paper chromatography of plant extract; thin layer chromatography (TLC)
of pharmaceutical drugs: To identify specific drugs using the technique
TLC; Column chromatography for protein separation/purification
6. Food Analysis: Determining the Vitamin C Content of Foods
Before and After Processing
Vitamin C determination of various samples, including fresh fruits
and other foods
7. DNA Technology
DNA Fingerprinting - Restriction Mapping and Forensics
Genetic Engineering - changing phenotype by changing genotype