Hemoglobin C protects against clinical Plasmodium falciparum malaria

DAVID MODIANO*†, GAIA LUONI*, BIENVENU SODIOMON SIRIMA‡, JACQUES SIMPORɧ, FEDERICA VERRA†, AMADOU KONATɇ, ELENA RASTRELLI*, ANNA OLIVIERI*, CARLO CALISSANO*, GIACOMO MARIA PAGANOTTI*, LEILA D'URBANO*, ISSA SANOUII, ALPHONSE SAWADOGOII, GUIDO MODIANO¶ & MARIO COLUZZI*†

* Dipartimento di Scienze di Sanità Pubblica, Sezione di Parassitologia, WHO Collaborating Centre for Malaria Epidemiology and Control; and
† Istituto Pasteur Fondazione Cenci Bolognetti, University of Rome "La Sapienza", 00185, Rome, Italy
‡ Centre National de Recherche et Formation sur le Paludisme, Ministère de la Santé; and
§ Centre Medical Saint-Camille; and
II Service de Pédiatrie, Centre Hospitalier National Yalgado Ouedraogo, Ouagadougou, 01 BP 2208, Burkina Faso
¶ Dipartimento di Biologia, Università "Tor Vergata", 00133, Rome, Italy

Correspondence and requests for materials should be addressed to D.M. (e-mail: david.modiano@uniroma1.it).

Hemoglobin C (HbC; b6Glu ® Lys) is common in malarious areas of West Africa, especially in Burkina Faso. Conclusive evidence exists on the protective role against severe malaria of hemoglobin S (HbS; b6Glu ® Val) heterozygosity, whereas conflicting results for the HbC trait have been reported and no epidemiological data exist on the possible role of the HbCC genotype. In vitro studies suggested that HbCC erythrocytes fail to support the growth of P. falciparum but HbC homozygotes with high P. falciparum parasitaemias have been observed. Here we show, in a large case–control study performed in Burkina Faso on 4,348 Mossi subjects, that HbC is associated with a 29% reduction in risk of clinical malaria in HbAC heterozygotes (P = 0.0008) and of 93% in HbCC homozygotes (P = 0.0011). These findings, together with the limited pathology of HbAC and HbCC compared to the severely disadvantaged HbSS and HbSC genotypes and the low bS gene frequency in the geographic epicentre of bC, support the hypothesis that, in the long term and in the absence of malaria control, HbC would replace HbS in central West Africa.